Gel Chromatography: Introduction, Theory, Instrumentation, and Applications

Gel chromatography, also known as size-exclusion chromatography (SEC), is a separation technique used primarily for separating molecules based on their size. It is widely used for purifying proteins, polysaccharides, and other macromolecules. In this technique, molecules are separated as they pass through a column filled with porous gel beads, and larger molecules elute (exit) from … Read more

Affinity Chromatography: Introduction, Theory, Types, Instrumentation and applications

Introduction to Affinity Chromatography Affinity chromatography is a type of liquid chromatography used for the separation, purification, or specific analysis of sample components. It is based on the principle of affinity, which refers to reversible biological interactions or molecular recognition between two molecules, typically an analyte and a ligand. These interactions are driven by the … Read more

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