ana Unit 3

Paper Chromatography: Introduction, methodology, development techniques, advantages, disadvantages and applications.

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Introduction Paper chromatography is a simple and effective analytical technique used to separate and identify mixtures of substances. This method is classified under partition chromatography, where separation occurs based on the differential partitioning of solutes between a mobile phase (solvent) and a stationary phase (paper). It was first introduced in the early 20th century and … Read more

Thin Layer Chromatography: Definition, Principle, Methodology, Types and Applications

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Thin Layer Chromatography (TLC) is a simple, quick, and cost-effective method used for separating and identifying compounds in a mixture. It is widely used in laboratories for qualitative analysis, especially in the fields of chemistry, biochemistry, and pharmacy. TLC helps to determine the purity of substances and monitor the progress of reactions. Principle of TLC … Read more

Partition Column Chromatography: Adsorption chromatography: Definition, Principle, Methodology, Types and Applications

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Partition column chromatography is a type of liquid-liquid chromatography where separation is based on the partitioning of components between two immiscible liquid phases—one as the stationary phase and the other as the mobile phase. This technique is commonly used for separating mixtures of compounds with varying solubilities and plays an important role in pharmaceutical, biochemical, … Read more

Adsorption chromatography: Adsorption chromatography: Definition, Principle, Methodology, Types and Applications

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Adsorption chromatography is a separation technique based on the differential adsorption of compounds onto a solid stationary phase. It is widely used to purify and separate chemical compounds based on their interaction with the adsorbent material. The process relies on the principle that different molecules have varying degrees of affinity to the surface of a … Read more

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